Abstract

DETECTION AND ESTIMATION OF FREE RADICAL SCAVENGERS QUERCETIN, RUTIN, GALLIC ACID AND CAFFEIC ACID IN SAFFRON BY HPTLC TECHNIQUE

Priyadharshini S., Sabitha S., Mukil M., Rajasekaran A. and Arivukkarasu R.*

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Abstract

Saffron is one of the most widely used spice in all over the world for its antioxidant property. The present study mainly aimed to detect the presence of flavonoids Quercetin, Rutin, Gallic acid and Caffeic acid in three marketed products of Saffron by HPTLC. The HPTLC method was performed using HPTLC aluminium sheets precoated with Silica Gel 60 GF254 as stationary phase and Ethyl acetate: Methanol: Water: Acetic acid (6.6: 2.3: 1.1: 0.7) as the mobile phase. The developed chromatogram was scanned at 254nm using Camag Scanner III. The Rf value of the standard Quercetin, Rutin, Gallic acid and Caffeic acid were found to be 0.84, 0.18, 0.76 and 0.85 The analysis outcomes confirm 0.12% of Quercetin in MEGSSC-2, 0.52% of Quercetin rationally more in MEMBSK-3 and 0.12% of Caffeic acid in MEKMS- 1 among the three marketed products of Saffron tested. The developed HPTLC method can be laboring for the routine investigations of flavonoids and phenolic acids. Among the three marketed product Kesaritatva was found to have more number of flavonoids.

Keywords: HPTLC, Saffron, Flavonoids, Antioxidant properties.