Abstract

CHEMICAL COMPOSITION, QUALITY CONTROL AND ANTIOXIDANT ACTIVITY OF SOME MARKETED CLOVE OIL SAMPLES AND POSSIBLE ANTI-COVID-19 ACTIVITY OF THE OIL

*Amira M. Beltagy

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Abstract

This work aimed to evaluate the quality of some commercial clove oil products in the Egyptian market either purchased or extracted from clove oil buds. Also, to evaluate the antioxidant potential of these oil samples. Thus quality control of theses oil samples depending on both results. Also, to test the antiviral clove oil activity against SARS-CoV as it was reported as one of the potent antioxidants and its major component; β- caryophellene showed activity against Covid-19. Seven essential oil samples were qualified depending on analyzed using GC/MS analysis by comparing the percentage composition of the main ingredients of clove oil; eugenol, eugenol acetate and β- caryophellene with those reported in pharmacopeia and other references. The antioxidant potential of these samples was performed using DPPH radical scavenging assay using ascorbic acid as a reference standard. The antioxidant potential of these samples had been taken in consideration for quality control of the tested samples. The antiviral activity of clove oil was assessed using Crystal violet assay. The tested samples showed variation either in the number of the peaks in the GC/MS chromatograms and thus was divided into three groups depending on similarity. Also, the percent concentration of each of the eugenol and other main ingredients showed variations. All the tested samples showed antioxidant potential with EC50 values ranging from 19.83 to 59.08 μg/ml. Clove oil showed activity against Coronavirus with SI equals 1.95 ng/ml.

Keywords: clove oil, GC/MS, quality control, antioxidant, anticovid-19.