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Abstract

REVIEW ARTICLE ON HIGH- PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Sudarshan Kejkar*, Neha Raut and M. Patel A. C.

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Abstract

High-performance liquid chromatography (or High pressure liquid chromatography, HPLC) is a specific form of column chromatography generally used in biochemistry and analysis to separate, identify, and quantify the active compounds. HPLC mainly utilizes a column that holds packing material (stationary phase), a pump that moves the mobile phase(s) through the column, and a detector that shows the retention times of the molecules. Retention time varies depending on the interactions between the stationary phase, the molecules being analyzed, and the solvent(s) used.

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