Abstract

BIOGENESIS, METHOD OF ISOLATION AND CHARACTERISTICS OF EXOSOMES

*Dipali Vaijinath Tidke, Dr. Nakul Kathar and Dr. Gajanan Sanap

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Abstract

Exosomes are small extracellular vesicles that are released by various cell types and play important roles in intercellular communication. Exosomes contain various biomolecules, such as proteins, lipids, and nucleic acids, that can be transferred between cells and modulate cellular processes. Exosomes have been implicated in various physiological and pathological processes, such as immune regulation, tissue repair, cancer progression, and neurodegeneration, and have potential applications as diagnostic or therapeutic agents. However, exosome research is plagued with the use of inconsistent isolation methodologies, nomenclature, and a lack of standardized data acquisition and analysis strategies. This limits the interpretation of the research conducted on exosomes. In this review, we explore the different methods for isolating exosomes and evaluate their advantages and disadvantages. We also discuss the challenges and opportunities in exosome research and highlight the need for standardized protocols and guidelines to ensure accurate and reproducible results.

Keywords: Exosomes, Isolation, Biology, Characteristics, extracellular vesicles.