Abstract

COMPARISON OF ANTIBACTERIAL ACTIVITY OF OCIMUM TENUIFLORUM L.AND PLECTRANTHUS AMBOINICUS (LOUR.) SPRENG AGAINST THE CLINICAL PATHOGENS STAPHYLOCOCCUS AUREUS, PSEUDOMONAS AERUGINOSA AND ESCHERICHIA-COLI

*Jiyauddin K., Samer A. D., Darashhni T., Rasha S., Jawad A., M. Kaleemullah, S. Budiasih, Rasny M. R., M. Qamar, Hamid K., Sakina R., Junainah A. H., Fadli A. and Eddy Y.

Abstract

Objective: The aim of this study was to identify and compare the antibacterial activity of Ocimum tenuiflorum and Plectranthus amboinicus leaves against the human gram positive and gram negative bacteria. Method: The research was conducted using the ethanol extract of Ocimum tenuiflorum and Plectranthus amboinicus. The antimicrobial activity of the extract was determined by using disc diffusion methods on the gram negative and gram positive bacteria. Results: By the observation, P. amboinicus shows higher antibacterial activity towards S. aureus, P. aeruginosa and E. coli at the concentration of 100 mg/mL which is 14mm, 12mm and 10mm respectively. Ocimum tenuiflorum also shows antibacterial activity at the concentration of 100 mg/mL S. aureus, P. aeruginosa and E. coli which are 12mm, 10mm and 10 mm respectively. Compared to Ocimum tenuiflorum, P. amboinicus shows higher antibacterial activity. Conclusion: There is a significant antibacterial activity of Ocimum tenuiflorum and Plectranthus amboinicus leaves against the selected human gram positive and gram negative bacteria.

Keywords: Ocimum tenuiflorum, Plectranthus amboinicus, Antibacterial and Essential oils.