Abstract

DETECTION AND ESTIMATION OF RUTIN, QUERCETIN, GALLIC ACID AND MANGIFERIN IN MELIACEAE, PIPERACEAE, AND ROSACEAE FAMILY BY HPTLC TECHNIQUE

Sabitha S., Naresh Kumar S., Mukil M., Rajasekaran A. and Arivukkarasu R.*

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Abstract

The prime aim of the study is to detect and estimate the flavonoids, and phenolic acids in commercial herbal raw materials namely Azadiracta indica flower, Azadiracta indica leaf, Ocimum santum leaf, Melia composita leaf, Piper cubeba bud, Piper nigrum bud, Rosa damacena, obtained from Meliaceae, Piperaceae, and Rosaceae species. We plan to confirm the presence of mentioned common secondary metabolites in herbal raw materials. Results of the study clearly revealed that these raw materials from Meliaceae, Piperaceae, and Rosaceae species contains flavonoids and phenolic acids. The developed HPTLC method shows the presence of rutin in Azadiracta indica flower, Azadiracta indica leaf, Melia composita leaf, Piper nigrum bud and it was estimated as 0.56%, 0.37%, 0.18%, and 0.02%. Gallic acid was estimated in Azadiracta indica leaf, Piper nigrum bud, and Rosa damacena, as 0.02%, 0.03% and 0.86% respectively. Quercetin and Mangiferin was predictable in all the raw materials and estimated less than 1% respectively.

Keywords: Quercetin, Gallic acid, Rutin, Mangiferin, HPTLC.