Abstract

GLYCOCONJUGATES-BINDING PROTEINS, GLYCOCONJUGATES FOR BIOTECHNOLOGICAL AND BIOMEDICAL USING. AN OVERVIEW OF OUR PUBLICATIONS

Lakhtin V. M.*, Lakhtin M. V., Aleshkin V. A. and Kombarova S. Y.

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Abstract

Our results of biotechnological aspects of the study of glycoconjugates (GC) and GC-binding proteins are briefly summarized: diagnostic and prognostic imaging and typing of systemic components of the C4 complement component of patients, including subisotyping; desialylation of GC on the example of glycoproteins; determination of soluble and immobilized biosurfactants and their pattern visualization on the example of culture fluids (CF) of Gram-positive bacteria (GPB),; determination of (glyco)proteins, including the determination of hydrophobic proteins and peptides of CF of GPB; determination of fluorophores in CF of GPB; preliminary analysis of the proteome of CF of GPB by mapping CF components separated by electrophoresis; determination of protease systems in CF of GPB on the example of probiotic strains of lactobacilli; blot block analysis of functionally active cytokines on the example of (glyco)hormone - erythropoietin; the use of horseradish peroxidase chemiluminescence, including the acetate-binding substrate of the catalytic center, in the study of systemic GC; determination of the blot block series of oxidoreductases in phyto- and microbial biological preparations of various degrees of purification, including biopreparations of medical importance; blot block determination of lectin and lectin-like proteins using synthetic polymer GC (www.lectinity.com); micropanel and blot block analysis of functionally active isotypes C4A and C4B and their subisotypes; system analysis of the relationship of microbial cell and metabolite antagonists in biofilms with or without participation of antibiotics.

Keywords: Glycoconjugates; glycoconjugates-binding proteins; gel electrophoresis; isoelectrofocusing; electroblotting; microanalysis; fluorescence; chemiluminescence; biological activities; functional activity of proteins; bacterial biosurfactants; systemic analysi