Abstract

EVALUATION OF THE COMBINED BACTERICIDAL ACTIVITY OF ESSENTIAL OILS OF PLECTRANTHUS GLANDULOSUS AND ECALYPTUS PF1

Mikolo Bertin*, Ampa Raoul, Baloki Ngoulou Tarcisse and Gatse Elgie Viennechie

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Abstract

Aim: The aim of this work was to test the sensitivity of 25 strains of Escherichia coli, Staphylococcus. aureus, Pseudomonas aeruginosa, Bacillus cereus, Salmonella sp., and Klebsiella pneumoniae to the essential oils of Eucalyptus PF1 and Plecthranthus galandulosus. Background: Plants of the Plectranthus and Eucalyptus genera are among the most used medicinal plants throughout the world. Their use constitutes one of the solutions to the treatment of bacteria resistant to current antibiotics. Objective: Identify which of the two essential oils or which of the mixture of the two essential oils or a mixture of the two oils makes it possible to maximize the diameter of the inhibition zone of the bacteria tested. Methods: The sensitivity of bacteria to essential oils was tested by the mixture design and the well diffusion method in three replicates. The regressions of the diameters of the inhibition zones were processed using the least squares method on Minitab. Results: Most of the strains tested were significantly inhibited by Eucalyptus PF1 and P. glandulosus. However, Ecalyptus oil was the most effective. Results allowed for validation of linear and quadratic regressions with 15 strains out of the 25 tested. Out of the fifteen optimizations carried out, eleven predicted maximum values of more than 14 mm with 100% Ecalyptus oil. Conclusion: The Eucalyptus PF1 essential oil was the best at killing strains of P. aeruginosa, B. cereus, and Staphylococcus.

Keywords: Plectranthus, Eucalyptus, Bacterial, Inhibition, Mixture.