Abstract

INVITROACTIVITIES OF MYRISTICA FRAGRANS LEAVES OIL AND ITS INHIBITS CARIOGENIC BIOFILM COLONIZER BACTERIANATURE’S GIFT IN ORAL HEALTH CARE

Dr. Sugithra B.*, Dr. Sundaramoorthi C., Anu Brindha K., Abinaya C. and Naresh Kumar S.

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Abstract

Dental caries and periodontal disease were associated with oral pathogens. Several plant derivatives have been evaluated with concerning their antiplaque effect against such pathogenic microorganisms. In this study, the pharmacological activity of myristica fragrans leaf oil and its effects on invitro dental Plaque biofilm studies. The volatile oil was isolated from the myristica fragrans leaf by using the steam distillation method (Clevenger apparatus). Methods used for evaluation of plaque were the Artificial dental biofilm model, Saliva conditioned flow cell model, Quartz crystal micro balance, and Zurich biofilm model have been reviewed. The VOMFL was evaluated for the inhibitory activity of the major cariogenic bacteria Streptococcus mutans, which has been generally regarded as the primary etiology agent of dental caries in humans, using an artificial dental biofilm model. The inhibitory actions were performed at a concentration of 1μl/ml and exposure of time to the test, standard, and were evaluated. The essential oil of leaves of myristica fragrans could be utilized for Pharmaceutical applications.

Keywords: Volatile Oil, Myristica fragrans leaf, Anti-Plaque, Biofilm, Nichrome wire.