Abstract

PHYTOCOGNOSTICAL, ANALYTICAL STUDIES, PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITIES OF SHOOTS AND ROOTS OF MIMOSA PUDICA LINN.

J. Priyananga*

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Abstract

The plant known as Mimosa pudica is found in Brazil and India. Across the tropics and subtropics of Asia, Africa, South America, North America, and Australia, the genus Mimosa is distributed. This genus has long been used to treat a wide range of conditions, including smallpox, hepatitis, tumors, HIV, ulcers, ringworm, asthma, leprosy, jaundice, diarrhea, fever, toothaches, wound healing, and skin and urinary issues. The Soxhlet extraction process uses water, methanol, chloroform, petroleum ether, and coarsely dried shoots and roots as the solvents. Within vacuum desiccators, the extract was concentrated. The transverse section histological analysis of the mimosa pudica root and shoot is carried out. The results of a quantitative physical examination were recorded for the shoot and root, including the total ash value, acid-insoluble ash, water-soluble ash, water-insoluble ash, watersoluble extract, alcohol-soluble extract, and ether-soluble extract. Alkaloids, polysaccharides, glycosides, and phytosterols have been identified by preliminary chemical investigation in MSME and MRME. MSME, MRME, MSCE, MRCE, MRWE, and MSWE are utilized as test compounds and DMSO is used as a blank when the gentamycin medicine was employed as a standard to determine the antibacterial activity. The disc diffusion method is used to measure the in-vitro antibacterial activity of the gram-positive bacteria Bacillus. When compared to the conventional gentamycin, MSME and MRME showed good antibacterial action against gram (+) ve bacterium bacillus. Clotrimazole is utilized as a standard, DMSO is used as a blank, and MSME, MSCE, MSWE, MRME, and MRWE are employed as test compounds in the case of antifungal activity. The disc diffusion method is used to measure the in-vitro antifungal activity of Aspergillus niger. Aspergillus niger does not exhibit any antifungal action from any of the test chemicals.

Keywords: Phytochemical screening, Disc diffusion method, Antibacterial, Anti-fungal.