Abstract

EVALUATION OF ANTIBACTERIAL ACTIVITY OF ZINGIBER OFFICINALE

Shiva Prasad Thoutu*

.

Abstract

This study was designed for evaluate the anti-bacterial activity found in association with bacterial diseases with Zingiber officinale. So many bacterial cultures available. I choose Escherichia coli. Inhibiting the bacterial activity by using Disc Diffusion Method the Kirby-Bauer method. Firstly, prepare the agar media with Peptone, Sodium chloride, Beef extract, Agar-agar and Water. After that prepare bacteria culture and swab in to the agar media. Using disc diffusion method after growing the E. coli it forms colonies in the Petri dish. Collect the organic Ginger powder and dissolve in DMSO extract dissolving in water and forming stock solution. Take the ginger solution apply it on the bacterial media. Few hours later slowly can observe inhibition of bacteria. Ginger extract had the overall widest inhibition E coli concentration. The results of different extracts were gained from extrapolation of zone diameter of inhibition of the concentration.

Keywords: Zingiber officinale, Anti-bacterial activity, Disc diffusion method, Escherichia coli, Inhibition.