Abstract

AMELIORATIVE EFFECT OF METHANOLIC EXTRACT OF MANIHOT ESCULENTA SEED ON ALLOXAN INDUCED DIABETIC PANCREATIC DAMAGE IN MALE WISTAR RATS

Chinecherem Onyekachi Muorah*, Dr. Uzozie Chikere Ofoego and Ikueze Ijeomma Chiamaka

.

Abstract

Diabetes mellitus presents a significant risk factor for the development of pancreatic injuries and is associated with both acute and chronic pancreatitis. This study investigated the effects of methanolic seed extract of Manihot esculenta (MSME) on body weight and pancreatic tissue in diabetic rats induced with alloxan monohydrate (AMH). Eight groups of seven animals each were assigned as follows: Group A received only distilled water and laboratory chow, Groups B and C received 100 mg/kg and 1000 mg/kg of MSME respectively for 3 weeks, Group D received a single dose of 120 mg/kg of AMH intraperitoneally, Groups E and F received AMH followed by immediate treatment with 100 mg/kg and 1000 mg/kg of MSME respectively for 3 weeks, Group G received MSME for 2 weeks followed by AMH induction. Thereafter, the animals were sacrificed after mild anesthesia and pancreas was harvested for histopathological studies. Data was analyzed using SPSS version 25 and results were expressed in mean ± SEM. Body weight and relative organ weight were analyzed using paired Sample T-test and One-way ANOVA (post Hoc LSD) respectively. Values were considered significant at P ≤ 0.05. Results indicated a significant increase in body weight in MSME-treated groups, particularly in Group F. Pancreatic tissue analysis revealed tissue regeneration and healing in MSME-treated groups, indicative of potential therapeutic effects on diabetes-related complications. These findings support the potential utility of MSME in managing diabetes and its associated complications. Further research is warranted to elucidate the underlying mechanisms and optimize treatment regimens.

Keywords: Manihot esculenta, Diabetic pancreatic damage, Alloxan-induced diabetes, Pancreatic tissue regeneration, Diabetes management.