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WJPR

World Journal of Pharmaceutical Research

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

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Abstract

BIOASSAY GUIDED ASSESSMENT OF FRACTIONS OF METHANOLIC EXTRACT OF CRINUM DEFIXUM AGAINST BRINE SHRIMP LETHALITY ASSAY

Subhashree Mohapatra and Sunita Bhatnagar*

Abstract

Methanol extract of leaf of Crinum defixum was subjected to extensive column chromatography, which resulted in 70 fractions. On the base of TLC analysis and brine shrimp assay, fractions were recombined in 13 major fractions. Majority of the fractions showed excellent cytotoxic activity more than 90%. Preparative TLC of one fraction resulted in a single fraction. On spectral analysis it consisted of one major peak and 3 small peaks.

Keywords: Methanol extract, Brine shrimp assay, Column chromatography, Crinum defixum.

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