VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF VALGANCICLOVIR IN BULK AND TABLET DOSAGE FORM
M.Lakshmi Surekha*, G.Kumara Swamy
Abstract
A simple, selective, linear, precise and accurate RP-HPLC method was
developed and validated for rapid assay of Valganciclovir
(valganciclovir) in bulk and in tablet dosage form. Isocratic elution at a
flow rate of 0.6ml/ min was employed on a Phenomenax Luna C18
column (150 4.6mm; 5) at ambient temperature. The mobile phase
consisted of Potassium di hydrogen phosphate buffer (pH-4.5) and
acetonitrile in the ratio 45:55. The PDA detection wavelength was 254
nm and 20 l of sample was injected. The retention time for
valganciclovir was 3.76 min. The method obeys Beer's law in the
concentration range of 20-60 g/ ml. The amount of Valganciclovir
Present in the formulation was found to be 99.60 1.5389. The %
recovery was in the range between 99.25% and 99.86%. The
percentage RSD for precision and accuracy of the method was found to
be less than 2 %. The method was validated as per the standard analytical procedures and
statistical parameters. The method was successfully applied for routine analysis of
valganciclovir in bulk and in formulation.
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