Abstract

GREEN SYNTHESIZED SILVER NANOPARTICLES USING TUBERS OF EULOPHIA SPECIES-PART I: OPTIMIZATION STUDY USING UV SPECTROPHOTOMETER

Patil Chandrashekhar J. and Patil Manisha C.*

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Abstract

The phytochemicals present in the plant extract readily act as reducing agents for the efficient conversion of metal salts or ions into metal or metal oxide nanoparticles. Flourishing applications of novel nanomaterials in biomedical and pharmaceutical fields encourage the biogenic synthesis of nanoparticles with reduced toxicity. As green synthesis of nanoparticles is simple mechanism, vast available, low cost, non-toxic, eco-friendly natured and one-pot synthesis method, it gains importance in the medical field, agriculture, and bioremediation. The various biological activities of Eulophia tubers are reported but no reports available on silver nanoparticles biosynthesis potential of Eulophia tuber extract. In this study, the phytoconstituents of tuber aqueous extract responsible for biosynthesis were analysed qualitatively. The process parameters for AgNP biosynthesis were optimized and studied using UV–Visible spectrophotometry. The increase in absorbance and the colour change, of the solution with time, quantity of extracts and various concentrations of AgNO3 were noted. The absorption optima of silver nanoparticles of E. herbacea and E. ochreata tuber aqueous extracts were obtained at wavelength 437 nm and 413 nm respectively at 1 ml extract quantity each for 5 h incubation at 60C. The phytochemicals present in the tuber extract played a significant role in the bio-reduction and stabilization of the synthesized AgNPs.

Keywords: Green synthesis, Silver Nanoparticles (AgNPs), Bio-reduction, Stabilization, Phytochemicals.