Abstract

A VALIDATED UV SPECTROPHOTOMETRIC METHOD DEVELOPMENT FOR CENTHAQUINE CITRATE IN BULK AND INJECTION DOSAGE FORM

Nagavalli D.*, Nishvanth F., Suganya K., Sujitha E., Supriya P., Thrisha S. and Yugapriya P.

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Abstract

Objectives: A simple, precise, accurate UV Spectrophotometric method developed for the determination of Centhaquine citrate in bulk and injection dosage form. Materials and Methods: The wavelength used for quantification was 232nm in distilled water. Beer’s law was obeyed at the concentration range 5-30μg/ml. The method is validated as per ICH guidelines. Stastical analysis proved that the method was accurate for centhaquine citrate. The wide linearity range, sensitivity, accuracy and simple procedure imply that the developed method can be used in the routine analysis of Centhaquine citrate in quality control laboratory. The different aliquots of Centhaquine citrate were prepared in the concentration range from 5-30μg/ml. The absorbance of the solution was measured at 232nm. The linearity procedure was repeated for six times. Results: From the linearity analysis, the calibration curve was plotted by using concentration against absorbance. The optical characteristics like Correlation Coefficient, slope, intercept were calculated. Conclusion: A validated UV spectrophotometric method development for Centhaquine citrate in bulk and formulated injection dosage form. The method was validated according to ICH guidelines with various parameters like Precision, Accuracy, Limit of detection, Limit of quantitation etc.

Keywords: Centhaquine citrate, Raw material, UV method and Injection dosage form.