Abstract

GENETIC ANALYSIS OF DRAGON FRUIT SELENICEREUS SPECIES

*V. Venkateshgoud and S. Karnakar Reddy

.

Abstract

Dragon fruit is considered an important economic fruit species worldwide due to its rich nutrient contents and strong to lerance to drought stress. In order investigate Genetic Analysis of Dragon fruits in Telangana 6 Germplasms were subjected to RAPD. The size of RAPD bands measured and score. The RAPD bands were scored as 1 if present and 0 if absent of bands of same molecular weight Five primers (Df series) amplified a total length of 39 loci with a size of 150-1400 bp. Amplified from the genomic DNA of Germplasm using 25 Decamer primers of random sequence were initially screened on DNA samples of 6 dragon fruit germplasm to evaluate their sustainability for amplifying DNA sequences, which could be accurately scored. A final subset of 5 primers. Dendrogram was generated by using NTSYSpc software that differentiated all the six dragon fruit samples. Respectively indicating Jaccard similarity coefficient. The similarity indices (SI) Vary from 0.11 to 0.88. The highest SI value (0.88) was found between Df-5, and Df-1 Germplasm whereas lowest observed between Df-6 and Df-3.

Keywords: RAPD MARKER, Monomorphism, Genetic Distance, Gene Analysis, Selenicereus species.