Abstract

DEVELOPMENT, CHARACTERIZATION AND EVALUATION OF POLYPHENOLIC PHYTO-PHOSPHOLIPID COMPLEXES (PHYTOSOMES) FOR IMPROVED SOLUBILITY AND SCAVENGING ACTIVITY

Pushpa Patiram Harijan, Ran Vijay Singh and Dr. Sanjay Kumar Kushwaha*

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Abstract

The aim of present study was to develop and characterise phytosomes of some herbal extracts to improve the solubility of its phytoconstituents. The poor solubility and dissolution rate of the phytoconstituents present in herbal extracts limit its oral absorption and bioavailability. The Standardised extracts of Citrus Limon (SCE), were used for development of phytosomes by solvent evaporation technique. The optimized phytosomes were characterized by various parameters like solubility studies, particle size determination, infrared absorption (FTIR), Differential scanning calorimetry (DSC), X-ray diffraction (XRD), Scanning electron microscopy (SEM), entrapment efficiency and in vitro antioxidant activity. SEM and XRD reveal the reduction in crystallinity of extract in the phytosome. FTIR and DSC confirm the formation of phyto-phospholipid complex. The apparent solubility and in vitro dissolution studies indicated a significant improvement in the solubility and the drug release of SCE, from phytosomes. It has been observed that thecomplex formed an effective scavenger of DPPH radicals. The result of the study revealed that the phytosomes may be considered as a promising drug delivery system that improves the solubility and absorption of plant constituents. As far as the potential of phytosome technology is concerned, it has a great future for use in formulation technology. Th e developed phytosomes in suitable dosage form may prove to be very effective in clinical use.

Keywords: Citrus Limon; phytosomes, DPPH, Antioxidant, SEM, In-vitro study Phospholipid, Phytosome, Polyphenolic, Herbal, Citrus lemon.