Abstract

GC-MS ANALYSIS OF METHANOL EXTRACT OF MILLETTIA PACHYCARPA ROOT

M. Premchand Singh, Lokhol Singh Sagolsem* and Yengkhom Sexona Singh

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Abstract

The plant Millettia pachycarpa belongs to genus Fabaceae which are distributed in the tropical and sub-tropical regions of the world. The present study on Gas Chromatography- Mass Spectrometry (GC-MS) analysis of methanol extract of the root of Millettia pachycarpa was carried out to determine qualitatively the presence of phytochemicals. In the GC-MS analysis, phytochemicals from the mass spectra which was matched with the National Institute of Standards and Technology (NIST), revealed the presence of 21 (Twenty one) compounds with their Retention Time. The dominant phytochemicals were Dibutyl phthalate (29.64%), Phthalic acid butyl hex-3-yl-ester (21.92%), Phthalic acid hept-4-yl isobutyl ester (17.31%), Phthalic acid butyl-3- methyl butyl ester (1.55%), Phthalic acid butyl hex-3-yl- ester(1.30%), Phthalic acid hept-4-yl isobutyl ester (1.28%), Phthalic acid pentyl-2- pentyl ester (1.26%), Phthalic acid bis (2-pentyl) ester (1.18%), Phthalic acid butyl hex-3-yl ester (0.84%) and Phthalic acid butyl isohexyl ester (0.81%). The phytochemicals like Ethane peroxoic acid, 1-cyano-1-[2-(2-phenyl-1,3-di-oxolan-2-yl) ethyl] (0.35%); phthalic acid di (2- methyl butyl) ester (0.28%); phthalic acid butyl-2-ethylbutyl ester (0.25%); phthalic acid, 3- methyl butyl pentyl ester (0.18%); 2-Butanone, 3-Chloro-4- hydroxy-1, 4-diphenyl (0.16%); (2-Bromo-1-propenyl), penta fluoro sulphur (0.14%); phthalic acid, 2- pentyl propyl ester (0.10%); phthalic acid, 2-methyl pent-3-yl-pentyl ester (0.08%); 2- cyclohexane-6-carboxylic acid, 3-methyl-1, 1, -(5-oxo, 3,4-di hydro-2Hpyrrolyl)- ethyl ester (0.07%); Tricyclo [4,1,0,0 (2,4) heptane, 5- (phenylthio) – (1a,2a,4a, 5a, 6a) (0.07%); 4-(Hydroxy methyl)-1-phenyl-2- azetidinone (0.06%), Acetophenone, 2-[(pnitrophenyl) imino] (0.05%); 2-Methyl-2-[(-1E, 3Z - E, 5E) 4 - methyl-6 - (2, 6, 6 - trimethyl - 1 - cyclohexenyl) -1, 3, 5 - hexatrienyl] - 1, 3 - dioxolane (0.05%); Silane, ethyl fluoro dimethyl (0.03%) and phthalic acid hept-1-yl isobutyl ester (0.03%) were found to be minor constituents. Further, these identified phytochemicals possess various biological activities such as antioxidant, antimicrobial, antibacterial, antifungal, anti-inflammatory, anticancer, blood tonics, anti- fertility, pesticides, plasticizer etc. Thus, these phytochemicals in the root of Millettia pachycarpa may help in the protection of human being from different kinds of diseases. The present finding highlighted the pharmaceutical importance of the phytochemicals present in the root of Millettia pachycarpa and further in depth analysis would be highly recommended for further studies.

Keywords: Millettia pachycarpa, GC-MS, Phytochemicals Biological activity, pharmaceutical.