Abstract

DEVELOPMENT AND VALIDATION OF UV VISIBLE SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF LAMOTRIGINE IN BULK AND PHARMACEUTICAL DOSAGE FORMS

*Malyala Swetha, Kannuri Sahithi, Mulukuntla Lahari, Pulinti Yashwanth, Mohammad Nabila and Sandeep Goud Mitta

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Abstract

Lamotrigine is an anticonvulsant drugs used in the treatment of epilepsy and bipolar disorder A simple, sensitive, accurate and reproducible UV/visible spectrophotometric method was developed for the determination of lamotrigine in bulk and pharmaceutical dosage forms. The solvent distilled water and wavelength corresponding to maximum absorbance for the drug was found at 300nm. Drug obeyed beer’s law in concentration range of 1to 5 ug/ml. With a correlation coefficient of 0.9990. The linear regression equation obtained was y =0.1371x+0.0168, where y is the absorbance and x is concentration of pure drug solution. The method was validated for several parameters such as Linearly, Accuracy, precision, Robustness as per ICH guidelines. The % recovery value with is close to 100% indicates reproducibility of the method and absence of interference of the excipients present in the formulation. The authors conclude that the proposed spectrophotometric method for the estimation of lamotrigine can be used for routine analysis of lamotrigine is bulk as well as in tablet dosage form.

Keywords: Lamotrigine, Dimethyl sulfoxide, Spectrophotometry.