Abstract

DEVELOPMENT AND VALIDATION OF UV/VISIBLE SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF LAMOTRIGINE IN BULK AND PHARMACEUTICAL FORMULATIONS

R. Navya, G. Sudheeksha, A. Thrisha, O. Anil Kumar, M. Nagamani and Raparthi Vijay Kumar*

Abstract

A simple, sensitive, accurate and reproducible UV/ visible Spectrophotometric method was developed for the determination of Lamotrigine in bulk and pharmaceutical dosage forms. The solvent used was acetonitrile and wavelength corresponding to maximum absorbance for the drug was found at 422nm. Drug obeyed beers law in the concentration range of 2-10μg/ml. with a correlation coefficient of 1. The linear regression equation obtained was y= 0.017x, where y is the absorbance and x is the concentration of the pure drug solution. The method was validated for several parameters such as linearity, accuracy, precision and robustness as per the ICH guidelines. The % recovery value which is close to 100% indicates reproducibility of the method and absence of interference of the excipients present in the formulation. The authors conclude that the proposed spectrophotometric method for the estimation of Lamotrigine can be used for routine analysis of Lamotrigine in bulk as well in tablet dosage form.

Keywords: Lamotrigine, spectroscope, absorbance, wavelength and ICH guidelines.