Abstract

PHYTOCHEMICAL INVESTIGATION, ANTIOXIDANT ACTIVITY AND ANTIHELMINTIC ACTIVITY OF MIKANIA MICRANTHA LEAVES

Ujjwal Kanti Dev, Md. Tanvir Hossain* and Md. Zahidul Islam

Abstract

In the present research investigation we extracted the powdered leaves of Mikania micrantha by Soxhletion method using different solvents. Then extracts were subjected to preliminary phytochemical investigation. Phytochemical screening of methanolic extract revealed the presence of alkaloids, flavonoids, reducing sugars, saponins, phenolic compounds and tannins, amino acids and proteins whereas petroleum ether extracts revealed only saponins, chloroform extract revealed alkaloids and saponins and water extracts revealed saponins and amino acid & proteins. The proximate analysis was carried out for the leaves powder. The total ash value was 5.31%, acid insoluble ash value was 4.77%, and water-soluble ash value was 4.15%. The materials were subjected to successive extraction with solvents. The solvents used were petroleum ether, chloroform, methanol and water in the ascending order of polarity. The total phenolic content was found 45.9 mg GAE/g extract. The IC50 values based on the DPPH for methanolic extract (41.8 μg/ml) showed lower than standard ascorbic acid (129.9 μg/ml) and BHA (153.1 μg/ml). The presence of phenolic compounds was mainly found in this extract and could be attributable to the observed high antiradical properties of this extract. The methanolic extract of Mikania micrantha has mild antihelmentic activity.

Keywords: M. micrantha, Proximate analysis, Phytochemical screening, Antioxidant, DPPH.