Abstract

ANALYTICAL METHOD VALIDATION FOR DOCOSAHEXAENOIC ACID CONTENT IN SURBEX PREGNANCY CAPSULES BY GAS CHROMATOGRAPHY

Keerthana S.*, Nalini C. N. and Vijayageetha R.

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Abstract

Fatty acid content in Surbex pregnancy capsules has frequently been measured using gas chromatography with flame ionization detection (GC-FID). The typical method for calculating the amount of docosahexaenoic acid (Omega-3-fatty acid) in Surbex Pregnancy Capsules was validated in this study employing GC-FID. Capillary, DB-23 (300X0.25X0.25)-Agilent was used to separate the DHA, and the entire run duration was 30.75 minutes. According to ICH criteria, the method’s linearity, precision, accuracy, specificity, and sensitivity were validated. Finally, this technique successfully and precisely identified and quantified omega-3 and fatty acids, and it may be applied to routine examination of Surbex Preganacy Capsules.

Keywords: Docosahexaenoic acid, GC Validation, Capillary, DB- 23 column ICH guidelines.