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Abstract

ORTHOGONALITY OF SEPARATION IN TWO DIMENSIONAL CHROMATOGRAPHY OF PLECTRANTHUS AMBOINICUS L. EXTRACT

Rasha Saad*, Fadli Asmani, Ameera Adeeba, Jiyauddin Khan, Mohammed Kaleemullah, Samer Al-dahlli, Rania Saad, Hamid Kazi and Eddy Yusuf

Abstract

Plectranthus amboinicus which from family Lamiacae and genus Plectranthus is traditionally used for the treatment of coughs, sore throat, asthma and disease affected by virus and bacteria. This plant is reported to have many biological activity such antiepileptic, antimutagenic, anti-inflammatory, anti-fungal and anti-tumor activity. Major chemical of this plants are carvacrol, thymol, a-terpineol, caryophylene oxide and B-seline. Although the plant showed an interesting bioassay, on the other hand, the extract was extremely complex for further purification. Thus the purpose of this research is to develop method to purify each of the active components. Two dimensional chromatography which refers to different selectivity between separation can improved the purification throughput complex mixtures. The plant was first extracted and then proceeds with column chromatography. By using column chromatography this plant was fractionated into 10 fractions by using methanol; methanol:chloroform and chloroform as mobile phase. Then, antioxidant activity was carried out for each of the fractions and extract using 1,1-diphenyl-2-picrylhydrazyl (DPPH) reagent. Finally all the fractions and extract were screening using Thin Layer Chromatography (TLC). The antioxidant activity of some fractions showed greater than the antioxidant activity of the extract. From the thin layer chromatography and HPLC, we found that they were possibly seven compounds. Three of them in the area of highly polar, two in the area of moderate polar and two of them in the area of low polar.

Keywords: Plectranthus amboinicus, two dimensional chromatography, fractionation, antioxidant activity, DPPH, methanol extract, column chromatography and thin layer chromatography.


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