Abstract

PHARMACEUTICAL STANDARDIZATION OF DRAKSHAGANDHA: A POLYHERBAL ARISHTA PREPARATION

Kamal A. Yeola*, R. S. Bhambar, J. Y. Nehete, A. Kulkarni, S. Pande and D. A. Karanjkar

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Abstract

Background: Drakshagandha is an ayurvedic polyherbal arishta formulation. It is necessary to carry out the standardization of Drakshagandha to ensure the safety and efficacy of it and to ensure the quality of finished product. The aim of the present work was to standardize the Drakshagandha in terms of organoleptic, physicochemical evaluation, spectroscopic and chromatographic techniques. Methods: Organoleptic analysis was carried out to determine the color, odor, and taste of formulation. In physicochemical evaluation Drakshagandha was analysed for various parameters such as pH, brix, specific gravity, alcohol content, total solid content, and total phenolic content. Spectroscopic (FTIR, UV spectroscopy) and chromatographic analysis (HPTLC) was done for identification and quantification of marker compound in Drakshagandha. Results: Drakshagandha was characterized as dark brown coloured liquid having sweet taste and aromatic odor. The pH of formulation was 4.17, Brix 34 %, Alcohol content 8.48 % v/v, Specific gravity 1.1037, Total solid content 35.13 % w/v, and Total phenolic content was 14.066 mg GAE/gm of Drakshagandha. FTIR and UV Spectroscopy reveal the unique spectra. HPTLC study showed major 5 spots for Drakshagandha. Gallic acid equivalent was identified by HPTLC at Rf 0.6 and was found to be 3.206 %. Conclusion: The results obtained in this research study can be employed for routine quality control analysis. Standardization ensures the consistent quality of Drakshagandha and reliable therapeutic outcomes.

Keywords: Drakshagandha, Gallic acid, Standardization, HPTLC, FTIR, UV Spectroscopy.