Abstract

OXIDOREDUCTASES AND LECTINS RECOGNIZING AND BINDING GLYCOCONJUGATES IN BIOPREPARATIONS ACCORDING TO CHEMILUMINESCENCE IN A REAL TIME

Vladimir M. Lakhtin*, Mikhail V. Lakhtin, Valery Y. Davydkin, Andrey V. Aleshkin, Vladimir A. Aleshkin

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Abstract

Oxidoreductases and lectins recognizing and binding glycoconjugates are value biologically active compounds and substances. So screening of oxidoreductases of probiotic and plant origin in any biopreparations of preventive and medical importance is actual one. The procedures for such type screening were developed. Systems of weakly acidic lactobacilli oxidoreductases (pI 5-5.6) and strongly acidic plant oxidoreductases (pI 3.2-3.7) were identified and registered. Oxidoreductases showed sensitivity to glycoconjugates and could coincide with the location of lectins. Highly sensitive methods of identification, purification, separation and strong immobilization on membranes of oxidoreductase systems using isoelectrofocusing in gel, electroblotting, chemiluminescence of substrate oxidation products in the mode of registration of stepwise kinetics of luminescence were investigated and proposed. Methods of identification and registration of both oxidoreductases and lectins (as coupled processes) are promising for further standardization of biological products, assessment of the presence of glycoconjugates in them, and the use of drugs as biosensors. In an isolated, immobilized form, oxidoreductase systems cofunctioning to lectins and glycoconjugates are promising for use as tools in microanalysis and biosensors of any exposed glycoconjugates.

Keywords: oxidoreductases; lectins; glycoconjugates; probiotics; phytopreparations; membranes; isoelectric focusing; electroblotting; chemiluminescence.