Abstract

GENETIC LINKED TO LEUKEMIA: ROLE OF POLYMORPHISMS IN CANDIDATE GENES INTERCONNECTED TO LEUKEMIA

Ali Hussein Al-Marzoqi*, Ammal Ragib Shemran, Raad Abbas Kadhim, Zainab Haider
Al-Mosawi, Lina Hassan Alasady, Worood Hamza Aldahmushe, Worood Alwan Al-Jeafry, Shahad Mohammed Darwal, Shahad Muhsen Hattem, Shahad Faddle Hashim

Abstract

Objective: To evaluate the overall progress and contribution of candidate gene association to the genetic susceptibility to Leukemia. Materials and methods:Study population included patients with cancer from Babil province - Iraq. Whole blood 60 cases at consultant clinic in Babil province from October 2014 to January 2015. Out of the 60 cancer patients, there were 37 male and 23 female. The patient's age range was from 25 to 70 years. Nominated mutations in CYP2E1 (G- 1259C, in linkage disequilibrium with C-1019T), in MPO (G-463A) and in NQO1 (C609T and C465T) were investigated by allele-specific oligonucleotide. Results: The frequencies among ALL patients differed from those of the control group. In particular, we found that carriers of the CYP2E1*5 variant were more frequent among cases (11.7% vs. 0% in controls), suggesting that the predicted higher level of CYP2E1 might be associated with an increased risk of LEUKEMIA. Similarly, it's revealed that NQO1 variants (NQO1*3) were associated with an increased risk of A LEUKEMIA, particularly due to a higher prevalence of heterozygous individuals among cases relative to controls. Conclusion: It's revealed that genetic polymorphisms in both NQO1 and CYP2E1 play a role in the development of LEUKEMIA. This is in accordance with the involvement of these variants in other types of leukemia.

Keywords: Leukemia, CYP2E1, MPO, NQO!, polymorphism, PCR, Iraq.