Abstract

PREPARATION AND CHARACTRIZATION OF PROPRANOLOLLOADED SOLID LIPID NANOPARTICLES FOR TOPICAL DELIVERY

Behzad Sh. Makhmalzade, Masoud A. Karami* and Azin Salehinezhad

Abstract

It was aimed to develop solid lipid nanoparticles (SLN) of propranolol using cold homogenization technique for topical delivery and to study its physicochemical properties. SLNs were prepared by different surfactants, different amounts of lipids and different concentrations of drug. Tween 20+Span 80, 1:1, or Lecithin were used as surfactants, cholesterol and oleic acid or compritol® and oleic acid were used as lipid phase. In vitro characterization of SLNs such as particle size and distribution, Differential Scanning Calorimetry study, drug entrapped efficiency and finally kinetic model of release of drug from formulations using phosphate buffer (pH=7.4) through cellulose acetate membrane were carried out. The results showed that the size of particles ranged from 15 to 84 nm which this allows drug-loaded particles to penetrate into skin easily and significantly. Drug entrapment efficiency for all formulations was 41 – 70% which is an acceptable range for propranolol as a hydophilic drug. Release of drug was less than 22% within the first 2 hours and more than 75% after 72 hours for the majority of formulations. DSC thermograms showed a phase transitional peak for each formulation in range of -11.5 to -47° C in cooling program and range of 60 – 117° C in heating program. Based on results, propranolol may be a candidate to be prepared as a topical solid lipid nanoparticles.

Keywords: Propranolol hydrochloride, Solid Lipid Nanoparticle, Topical drug delivery.