Abstract

EXPLORING NYCTANTHES ARBOR-TRISTIS BIOACTIVE COMPOUNDS AS NATURAL INHIBITORS OF ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE: IMPLICATIONS FOR TYPE 2 DIABETES MANAGEMENT

Akash P. Patil*, Pratiksha P. Patil, Chetan M. Patil, Digvijay D. Jadhav and Swapnali S. Jagtap

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Abstract

Background: Nyctanthes arbor-tristis, known for its pharmacological diversity, is increasingly studied for its potential therapeutic benefits, including in type 2 diabetes management. Type 2 diabetes mellitus, characterized by insulin resistance and dysregulated glucose metabolism, necessitates effective treatment options to mitigate its health impacts. Natural products, like those from Nyctanthes arbortristis, present promising avenues due to their bioactive compounds potentially acting as enzyme inhibitors. Methodology: This study employed molecular docking to screen 30 biomolecules derived from Nyctanthes arbor-tristis against alpha-amylase and alpha-glucosidase, key enzymes in glucose metabolism. Molecular docking simulations were conducted to predict ligand binding affinities and interactions with enzyme active sites. The computational approach facilitated the assessment of these compounds' potential to modulate enzyme activity, crucial for managing type 2 diabetes. Results: Six bioactive compounds Lupeol, Beta-Sitosterol, Oleanolic Acid, Nicotiflorin, iridoid, Astragalin, demonstrated significant binding affinities and interactions with alphaamylase and alpha-glucosidase. Notably, interactions such as hydrogen bonding and hydrophobic interactions were observed, indicative of effective enzyme inhibition mechanisms. These findings underscore the potential of Nyctanthes arbor-tristis compounds as inhibitors of carbohydrate-digesting enzymes, suggesting their utility in regulating blood glucose levels. Conclusion: This research highlights Nyctanthes arbor-tristis as a promising source of natural inhibitors for alpha-amylase and alpha-glucosidase, pivotal in glucose metabolism regulation. The identified bioactive compounds show potential for developing novel therapeutic agents for type 2 diabetes. Further studies are essential to validate these findings experimentally and explore their clinical applications. This study contributes valuable insights into utilizing natural products in drug discovery, emphasizing the need for continued investigation into botanical sources for innovative diabetes treatments.

Keywords: Diabetes mellitus, Nyctanthes arbor-tristis, alpha-Amylase, Molecular docking.