Abstract

PREPARATION AND EVALUATION OF MUCOADHESIVE GEL OF MORINGA OLEIFERA

Sahil Srivastava*, Amit Bind, Jwala Yadav, Rishabh Srivastava, Amit Chaturvedee, Ankit Sharma

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Abstract

In the past few decades, a great deal of in vitro and in vivo research has been done to examine the efficacy of medicinal plants in treating periodontal. Strongly effective as a medicine, moringa oleifera has anti-inflammatory and immunomodulatory qualities. Our study’s objectives are to plan, formulate, and assess M. oleifer diseases a extract’s antibacterial efficacy for local drug delivery (LDD) as a periodontal treatment. Materials and Methods: An in vitro experimental model was used in this work. M. oleifera powdered dried leaves and 70% ethanol were macerated to create M. oleifera extract. The broth dilution method was utilized to determine the minimum inhibitory concentration (MIC) of Moringa extract against Porphyromonas gingivalis. The obtained MIC of the Moringa extract was used to prepare the gel along with a mixture of polymers, specifically polyethylene glycol 6000, carbopol 940, and chitosan. The prepared gel was also put through syringeability testing, pH measurements, and thermodynamic stability tests for in vitro characterization. With the aid of the Brookfield DV-II + Viscometer, viscosity was measured. A manufactured mucoadhesive strength test device was used to measure mucoadhesive strength. Result: M. oleifera leaf extract is a powerful botanical extract for the formulation of LDD agents for periodontal diseases because it exhibits a bactericidal effect against P. gingivalis even at low concentrations of 25μg/ml. After adding the herbal extract to the blank gel, the formulation demonstrates sufficient stability, good mucoadhesiveness, and controlled drug release. Conclusion: The keystone pathogen in the etiopathogenesis of periodontitis, P. gingivalis, has been shown to be susceptible to the bactericidal effect of the M. oleifera leaf extract. Therefore, as an LDD agent, extract from M. oleifera leaves can be used to treat periodontal diseases.

Keywords: Local drug delivery; Moringa oleifera; Porphyromonas gingivalisa; mucoadhesive gel; periodontal disease.