Abstract

PHYTOCHEMICAL SCREENING AND INVITRO EVALUATION OF ANTI BACTERIAL ACTIVITY OF OCIMUM SANCTUM AGAINST E. COLI.

Shivangi Srivastava*, Shishu Pratap Singh and Shantanu Kushwaha

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Abstract

This study investigates the phytochemical composition and antibacterial activity of Ocimum sanctum (Tulsi) against Escherichia coli. Recognized for its extensive medicinal properties, Tulsi has been utilized in traditional medicine across various cultures. The research involved the collection and preparation of Tulsi leaves, followed by phytochemical screening to identify secondary metabolites such as saponins, tannins, alkaloids, carbohydrates, and flavonoids. The antibacterial efficacy of the leaf extract was evaluated using the disc diffusion method on Muller Hinton Agar, with results indicating a measurable zone of inhibition against E. coli. The findings suggest that Ocimum sanctum possesses significant antibacterial properties, supporting its traditional use in treating infections and promoting health.

Keywords: Ocimum sanctum, Tulsi, Phytochemical screening, Antibacterial activity, Escherichia coli, Medicinal properties, Traditional medicine, Secondary metabolites, Disc diffusion method, Herbal medicine, Antibiotic resistance, Natural antibacterial agents, Thera