Abstract

STUDY OF COMBINED EFFECT OF SEDATIVE ANTIHISTAMINES AND PARACETAMOL ON ANIMAL BEHAVIORS

Noor Saba and Raziya Begum*

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Abstract

Sedating antihistamines, known for their high-fat solubility and ability to cross the blood-brain barrier, are commonly used to alleviate conditions such as dermatitis-induced itching, which can cause sleep disturbances. However, their strong antimuscarinic effects necessitate caution in patients with prostatic hypertrophy, urinary retention, and angle-closure glaucoma. This study aimed to assess the sedative effects of cyproheptadine (2.5 mg/kg), chlorpheniramine (2 mg/kg), and ketotifen (1 mg/kg) on mice, using a range of behavioral and physical tests including the Rota rod, Actophotometer, Plus maze, and Open field tests. Male and female mice were divided into seven groups (n=7) and treated over four days. Additionally, the sedative effects of these antihistamines were evaluated in combination with 10 mg/kg paracetamol. Parameters such as ambulation, rearing, preening, defecation (Open field), entries into open and closed arms (Plus maze), and percentage changes in time (Rota rod) were measured. The results indicated that the combination of sedating antihistamines with paracetamol significantly reduced the sedative adverse effects of the antihistamines. This beneficial effect may be attributed to the blockade of H1 receptors or decreased histamine release. The findings suggest that this combination could offer a promising therapeutic option for managing allergies. Further investigations are necessary to elucidate the underlying mechanisms and to confirm these effects in human clinical studies.

Keywords: Allergy, Anti-Histamine, H1 receptors, Paracetamol, Sedative.