Abstract

IN-VITRO ANTI-INFLAMMATORY ACTIVITY OF KAARA SOODA SATHU PARPAM (KSSP) – A SIDDHA HERBO MINERAL FORMULATION BY PROTEIN (ALBUMIN) DENATURATION

Murugalakshmi R.*, Powrna V., Jani Antony S. and Anbu N.

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Abstract

Inflammation is a complex biological response that involves various molecular mechanisms, including protein denaturation. Uncontrolled inflammation can lead to chronic diseases, current synthetic antiinflammatory drugs have side effects. Therefore, there is a need for potent anti-inflammatory agents from medicinal plants. Kaara Sooda Sathu Parpam (KSSP) is a Siddha Herbo mineral formulation used in traditional medicine to treat inflammatory disorders. This study aimed to evaluate the in vitro anti-inflammatory activity of KSSP by investigating its ability to inhibit protein (albumin) denaturation. The results showed that KSSP significantly inhibited heat-induced albumin denaturation, with a maximum inhibition of 47.9% at 500 μg/ml, comparable to the standard anti-inflammatory drug diclofenac sodium (92.59% at 100 μg/ml). The findings of this study suggest that KSSP's anti-inflammatory activity may be attributed to its ability to inhibit protein denaturation, a key event in the inflammatory cascade. These results support the traditional use of KSSP in Siddha medicine and highlight its potential as a therapeutic agent for inflammatory disorders. Further investigation is warranted to fully understand KSSP's anti-inflammatory mechanisms and therapeutic potential. This study provides a foundation for the development of KSSP as a novel antiinflammatory agent, offering a promising alternative to synthetic drugs.

Keywords: Kaara Sooda Sathu Parpam (KSSP), Siddha Herbo mineral formulation, Antiinflammatory activity, Protein denaturation, Albumin, Inflammation, Traditional medicine, Diclofenac sodium, In vitro study, Therapeutic potential.