Abstract

INVESTIGATING AND COMPARING THE SUPPRESSIVE EFFECT OF A POLYHERBAL FORMULATION (PHF) BY USING INVITRO ANTICANCEROUS CELL LINES AND DOCKING STUDIES

*Dr. S. Yamini Sudha Lakshmi, V. Yavanika, Dr. S. Subramanian

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Abstract

Herbal medicinal plants have been investigated for various pharmacological properties, yet many phytoconstituents remain unexplored. A polyherbal formulation (PHF) was developed by homogeneously mixing a specific proportion of Justicia adhatoda, Glycyrhiza glabra, Withania somnifera, Cassia angustifolia, Zingiber officinale, Elletaria cardamomum, Terminalia chebula, Nigella sativa, Curcuma longa, Piper nigrum, Moringa oleifera, Cuminum cyminum, Phyllanthus emblica, Tribulus terrestris, Andrographis paniculata, Cinnamomum verum, Tinospora cordifolia, Gymnema sylvestre, Abieswebna, Vettiveria zizanoides, Ociumum sanctum, Centella asiatica, Trigonellafoenum-graecum, Solanum triblobatum, Azadirachta indica leaves. This study aims to assess the anticancer properties of Methanolic extract of PHF on breast (MCF7), colorectal (HT29), lung (L2), liver (HepG2), and renal (MDCK) cancer cell lines, as well as its drug-likeness properties through insilico analysis. The methanol extract of PHF was tested for cytotoxic activity using the MTT assay at concentrations ranging from 1000 to 15 μg/ml. The results showed that the compound inhibits the colorectal (HT29) cell line with IC50 values of 97.24 ± 62.04, more effectively than the other four cell lines. Comparatively, the compound isolated from methanolic extract of PHF in previous study demonstrated the best results against colorectal cancer. In the molecular docking investigation, the compound 1,1-Bis(hydroxymethyl)cyclopropane was selected for its interaction with proteins such as Cdk2, TRAF, nitric oxide synthase, Bcl, and Chk1, which are involved in colon cancer. According to molecular docking and ADMET data, CDK2 exhibited the highest binding energy scores with the compound. These findings suggest that it might be effective against colorectal cancer and further studies using animal model might confirm its anticancerous activity against colorectal cancer.

Keywords: PHF, cell line, Colorectal, molecular docking.