Abstract

PHARMACOLOGICAL EVALUATION AND COMPARISON OF ANTI-EPILEPTIC ACTIVITY OF OCIMUM BASILICUM LINN AND OCIMUM SANCTUM LINN IN ALBINO RATS

Mansi Gupta*, Amrita Singh, Snehil Singh and Bhanu P. S. Sagar

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Abstract

Present work was envisaged to perform research on pharmacological investigations to assess and compare pharmacological activities of Ocimum basilicum Linn. (Sweet basil) and Ocimum sanctum Linn. (Shyama/holy basil) in albino rats. PPMs and SPMs such as phenolics, flavonoids, alkaloids, proteins, tannins, steroids, glycosides, terpenes, amino acids, carbohydrates, and reducing sugars were found in the qualitative analysis of EEOBL and EEOSL leaves extracts. High concentration of TFC was found in EEOBL extract in comparison of EEOBL extract. In Physico-chemical analysis, high water soluble and ethanol soluble extractive values indicated that leaves powders contain water soluble salts. The fluorescence analysis indicated that leaves powders contains high concentration of phenolic and flavonois as SPMs. EEOBL and EEOSL extracts caused no significant change blood parameters and LFTs. The EEOBL and EEOSL extracts induced a very wide safety margin (no mortality upto 2000 mg). Finally, in invivo anti-epileptic activity, EEOSL-B extract was found to be more effective than EEOSL-B but less effective than standard drug Phenytoin / Saraswat Syrup. Finally, combination of EEOBL-B and EEOSL-B very significantly decreased convulsion. Much more elaborated pharmacological research is proposed.

Keywords: Anti-epileptic activity, Antioxidant, Ocimum basilicum, Ocimum sanctum, phenytoin, saraswat syrup, secondary plant metabolites, acute toxicity, sub-acute toxicity, shyama / holy basil, sweet basil.