Abstract

ANTIHYPERGLYCEMIC AND ANTIHYPERLIPIDEMIC EFFECTS OF DIASHIS, A POLYHERBAL FORMULATION, IN STREPTOZOTOCININDUCED DIABETIC MALE ALBINO RAT: AN APPROACH THROUGH TOXICITY STUDY

Tushar Kanti Bera , Kishalay Jana1, Soumyajit Maity, Abhinandan Ghosh, Sanjit Kumar Kar, Debidas Ghosh*

Abstract

To investigate the mode of antidiabetic and antihyperlipidemic activity of Diashis, in streptozotocin (STZ)-induced diabetic male albino rats. Hyperglycemia was induced by single intramuscular injection of STZ at the dose of 4 mg / 0.1 mL of citrate buffer (pH 4.5) / 100 g body weight. For acute toxicity evaluation of Diashis, the formulation was administered orally at the doses ranging from 2.5, 5, 10, 20, 40, 80, 160, 320 mg / 100 g body weight. Significant diminution in the activities of hepatic hexokinase and glucose-6-phosphate dehydrogenase along with elevation in glucose-6-phosphatase were noted in STZ-induced diabetic animals. Levels of fasting blood glucose (FBG), glycated hemoglobin (HbA1C), serum lipid profiles were elevated significantly (P<0.001) along with the diminution in serum insulin level in STZ-induced diabetic animals. Above mentioned parameters were recovered significantly (P<0.01) towards the control level after treatment of Diasihs for 30 days at the dose of 5 mg / 0.5 ml distilled water / 100 g body weight / rat / day at fasting state to diabetic animals. In an acute toxicity study, there was no toxic symptom up to the dose level of 320 mg / 100 g body weight. From these results, it may be concluded that Diashis at the dose of 5 mg / 100 g body weight is safe for long term treatment for diabetic protection.

Keywords: Diashis, Insulin, Lipid profile, Streptozotocin.