Abstract

A COMPREHENSIVE REVIEW ON HPLC ANALYTICAL METHOD

Maithili Dhond*, Punam Kelkar, Ashlesha Bhogate, Suyash Mandavkar, Sushant Kadam and Amankumar Chavan

Abstract

HPLC (High Performance Liquid Chromatography) is a type of column chromatography. It is analytical technique which is used to examine solution that is eluting from column. It is also one of the most powerful technique for separation and quantitative determination of component in mixture. Most of the drug that present in multicomponent dosage for can be analyze by HPLC method. It is work on principle that involve two phases: “Stationary Phase” and “Mobile Phase”. According to ICH Guidelines, validation of HPLC process includes testing for system appropriateness, accuracy, precision, specificity, linearity, range, limit of detection and quantification, robustness and other factors.

Keywords: HPLC.