Abstract

EXPLORING CHROMATOGRAPHIC TECHNIQUE: PRINCIPLE, INSTRUMENTATION AND ITS APPLICATION

Trupti Jagtap*, Muskan Mujawar, Sabiya Mulani, Abhijit Mande and Omkar Gaikwad

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Abstract

This review covers the fundamentals and applications of chromatographic techniques in drugs specifically HPLC, GC, TLC, ion exchange chromatography and Column Chromatography. Their mechanisms of separation and their application in drug development formulation analysis pharmacokinetic studies and biopharmaceutical characterization are also discussed. A comprehensive overview of the critical influencing factors in chromatographic performance, including mobile-phase composition, stationary-phase characteristics, operating conditions, detector sensitivity, and sample preparation methods, which are essential to obtaining robust results for analysis, is also included.

Keywords: Their mechanisms of separation and their application in drug development formulation analysis pharmacokinetic studies and biopharmaceutical characterization are also discussed.