Abstract

CHROMATOGRAPHIC MASTERY: HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) TECHNIQUE

Nehe Ashvini* and Khatal Pradip

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Abstract

High performance liquid chromatography (HPLC) is a type of column chromatography which is commonly used in biochemistry and analysis to separate or identify & quantify the active chemicals. HPLC is the most used separation technology for detecting, separating & quantifying the drugs. The accuracy, specificity, linearity, range, limit of detection, the limit of quantification, robustness, and system suitability testing are all included in the validation of an HPLC techniques according to ICH guidelines. Chromatography is used in all research laboratories and pharmaceuticals industries universally. The chromatography Is very popular techniques and it is mostly used analytically. There are different types of chromatography techniques namely paper chromatography, gas chromatography, liquid chromatography, thin layer chromatography (TLC), ion exchange chromatography and lastly high-performance liquid chromatography (HPLC). This review article mainly focuses on the chromatography types and HPLC, parameters of HPLC, instrumentation of HPLC, validation method, pump, detectors and types of detectors, application of HPLC.

Keywords: Chromatography, mobile phase, stationary phase, analyte, HPLC, validation, pump, detectors.