Abstract

HEPATOPROTECTIVE EFFICACY OF METHANOLIC EXTRACT OF LEAVES OF CISSAMPELOS PAREIRA IN ACETAMINOPHEN INDUCED TOXICITY ON HEP G2 CELLS

Latha B.* and Lekshmi Venugopal

Abstract

Objectives: To investigate the hepatoprotective efficacy of most active extract of leaves of Cissampelos pareira in acetaminophen induced toxicity on Hep G2 cell lines. Materials and Methods: Phytochemical studies were carried out using three different solvents like hexane, ethyl acetate and methanol. The antioxidant activity of different extracts was studied by in vitro assay using DPPH and IC50 value was determined. HPTLC analysis was also carried out. Hepatoprotective efficacy of methanolic extract of C. pareira in acetaminophen induced Hep G2cell lines were analysed using MTT assay. Results: Preliminary phytochemical analysis revealed the presence of phenol, flavonoid, alkaloid, glycosides, saponins, steroids, carbohydrates, proteins and aminoacids. From the different extracts methanolic extract showed high antioxidant activity. HPTLC analysis of methanolic extract showed the presence of flavonoid. The cytotoxic studies revealed that methanolic extract of leaves of Cissampelos pareira at a concentration of 12.5μg/ml showed maximum hepatoprotective action in acetaminophen induced Hep G2 cells. Conclusion: The results suggest a hepatoprotective action for methanolic extract of leaves of Cissampelos pareira in acetaminophen induced Hep G2 cells due to its antioxidant properties.

Keywords: Hepatoprotection, Cytotoxicity, Acetaminophen, Hep G2 cells, Cissampelos pareira.