Abstract

QUANTITATIVE ANALYSIS OF TOTAL PHENOLIC CONTENT IN BANAFSHAH FLOWER (VIOLA ODORATA. LINN) EXTRACTS

Abdul Wahab*, Aisha Siddiqui, Umar Jahngir, Ayman Kirmani, Anwar Jamal and Rasikh Javaid

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Abstract

Viola odorata flower were analyzed for total phenolic contents. This phenolic component is responsible for antioxidant activity. The amount of total phenols were analyzed with the Folin-Ciocalteu reagent. Gallic acid was used as a standard compound and the total phenols were expressed as mg/g gallic acid equivalents (Standard curve equation: y = 0.0507 + 0.0619, R2= 0.9689). The hydroalcoholic extract had a lower TPC value of 11.496±0.207 mg/g, indicating that the aqueous extract contains a higher concentration of phenolic compounds. The total phenolic content of the aqueous extract was found to be 22.163 ±0.5356 mg/g.

Keywords: Viola odorata, total phenols, Folin-Ciocalteu reagent, Gallic acid.