Abstract

SCREENING OF LUPEOL ON PARTIAL SCIATIC NERVE LIGATION INDUCED NEUROPATHIC PAIN IN WISTAR RAT

*Gaikwad Ashlesha S., Kamble H. V., Ghodake S. R. and Bhintade Charushila

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Abstract

The current study focuses on evaluating the effects of Lupeol on neuropathic pain induced by partial sciatic nerve ligation (PSNL) in Wistar rats. Neuropathic pain, characterized by sensory dysfunction and chronic pain, significantly impairs quality of life. The research aims to induce neuropathic pain using PSNL in rats and assess pain using various behavioural and biochemical parameters, including the Von Frey filament test (mechanical allodynia), Inclined Plane test (muscle strength), Hot Plate test (thermal hyperalgesia), and Randall- Sellito test (pain threshold). Additionally, biochemical markers and histological analyses will provide insight into nerve injury and neuroinflammation. The study builds on Lupeol’s known antiinflammatory, anti-arthritic, and neuroprotective properties, exploring its potential role in modulating neuropathic pain through peripheral and central sensitization mechanisms. Lupeol is hypothesized to reduce pain by inhibiting neuroinflammation, peripheral sensitization, and promoting neuroplasticity, offering a promising therapeutic approach to neuropathic pain management.

Keywords: Lupeol, Neuropathic pain, hyperalgesia, sciatic nerve, allodynia.