Abstract

CHEMILUMINESCENT TECHNOLOGY IN A LIVE IMAGE FOR DETECTING THE BLOOD BIOMARKERS OF AUTOIMMUNE DISEASES: BIOMARKER OF LESION DEPTH

Lakhtin V. M.*, Lakhtin M. V. and Aleshkin V. A.

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Abstract

An analysis was carried out and a technology was proposed in connection with the use of complement components to identify biomarkers of autoimmune diseases. The technology of detection of a biomarker of systemic lupus erythematosus (SLE) - subisotype C4A5- pI-3,8-BLOT-pH7 of the complement system component C4 in the serum of patients is evaluated. The uniqueness of the identified subisotype lies in the indication of the depth of the patient's lesion with SLE against the background of antiphospholipid syndrome (APS). The prospects of biomarker subisotypes involving the complement system are discussed.

Keywords: Real-time chemiluminescence, isoelectrofocusing in polyacrylamide gel, electric immunoblotting, immune enzyme analysis (IEA), components of C4A and C4B, biomarkers, systemic lupus erythematosus (SLE), antiphospholipid syndrome (APS).