Abstract

HYPOGLYCEMIC ACTIVITY OF LYOPHILIZED TULSI LEAF POWDER AND ITS PROTECTIVE ROLE ASSOCIATION WITH THE ANTIOXIDANT AND ANTIDYSLIPIDEMIC PROPERTIES IN ALLOXAN-INDUCED DIABETIC RATS

Umadevi C. Jadaramkunti*

Abstract

This study was aimed to assess the antidiabetic and antihyperlipidaemic potentials of lyophilized tulsi leaf powder (LTLP) and in correlation, with standard drug glibenclamide (2.5 mg/kg BW/day) on alloxan monohydrate induced diabetic male albino rats. Diabetes was induced in Wistar rats by the administration of alloxan monohydrate at 100 mg/kg of body weight. After 48 hrs, rats with fasting blood glucose levels of 200 mg/dL and above were taken into consideration diabetic and used for the study. Wistar albino rats were divided into five groups of 10 animal each viz. Group I rats nondiabetic (normal control), Group II consisted of diabetic control rats that received no treatment, Group III rats diabetic conventional treated (with Glibenclamide) and Groups of IV and V rats diabetic dealt with (supplementation with graded doses of LTLP, 50 and 100mg/ kg, BW/ day, respectively). Blood samples were collected at 28 post-treatment and blood glucose, relative body weight, serum biochemical parameters insulin, glucose, urea, creatinine and glycosylated haemoglobin (HbA1c), protein, albumin, globulin, SGPT, SGOT, ALP AST and ALT, lipid profile (TC, TG, LDL-C, VLDL-C, HDL-C, and phospholipids). In addition, to evaluate the protective potentials of LTLP, the pancreas and liver and tissues were collected, and tissues homogenate were prepared to analyse for the assessment of activities of oxidative stress parameters (total protein, MDA, GSH, GST, SOD and catalase activities) in diabetic induced rats were performed. The results established that graded doses of LTLP supplementation in alloxan induced diabetic rats significantly (P ≤ 0.05) altered blood glucose levels, HbA1c and other biochemical parameters level were observed in diabetic rats compared to diabetic control rats. Treatment with LTLP produced significant (P ≤ 0.05) recovery in the levels of lipid profiles towards the control levels when compared with diabetic control and glibenclamide drug. These findings showed clearly indicates that carbohydrate nature of LTLP has an important antidiabetic, antidyslipidemic, and antioxidant potential.

Keywords: LTLP, Antidiabetic, Antioxidant Antihyperlipidaemic, Alloxan, Glibenclamide, Serum biochemical indices.