Abstract

SIMULTANEOUS DETERMINATION OF ARGATROBAN AND ZONISAMIDE BY RP – HPLC METHOD

*Abhishek Kumar B. Shah, Dr. Rakesh Kumar Jat and Dr. Hasumati A. Raj

Abstract

UV and HPLC Method was developed and validated for Argatroban, anticoagulant and anticonvulsant drug zonisamide used to treat Alzheimer’s Disease. High-performance Liquid Chromatography method as per ICH Q2R1 guidelines has been developed for determination of Zonisamide and Argatroban in synthetic mixture. Chromatographic separation was archived with Phenomax Kinetex ® (C18, 5μ, 250 x 4.5 mm) column. As a mobile phase mixture of Acetonitrile, Methanol and Water in the ratio of 60:25+15 v/v/v with pH 6.8 adjusted with OPA was used. Drugs were analysed at 277 nm wavelength with 1 mL/min flow rate. HPLC method was developed and all the results of linearity, accuracy, precision proved to be within limits with LOD and LOQ. Robustness of method was confirmed as no significant difference was detected on analysis by subjecting the method to slight change in the method condition. % Assay was 101.00 ± 0.015 and 100.76 ± 0.097 for Zonisamide and Argatroban respectively.

Keywords: Zonisamide, Argatroban, HPLC method.