Abstract

ISOLATION AND CULTURE OF ACINETOBACTER BAUMANNII

Sahira Nsayif Muslim, *Shatha Ali Shafiq, Mohammed Fadhel Khudhair

Department of Biology, College of Science, Mustansiryia University, Iraq.

Abstract

The present work deals with physiological studies on bacterial Lasparaginase. It includes studies on the productivity of L-asparaginase by different Acinetobacter baumannii is isolated and cell immobilization. Six isolates under shaken condition were investigated for their abilities to produce L-asparaginase enzyme from them Acinetobacter baumannii S4 isolated from sputum, samples gave the highest production level of enzyme. The maximum activity ( 46.70 μ /ml) of L-asparaginase was obtained using M9 medium supplemented with 0.5% soluble starch as carbon source and 0.5% L-asparaginase as nitrogen source , with initial pH 8 , inoculated with 2 ml of 26 h old culture and incubated at 37 ºC for 26 h . Cell immobilization was recorded by physical immobilization and sawdust was the most favorable for the production of the highest L-asparaginase activity 41.2 μ / ml. Entrapment immobilization was the most suitable with sodium alginate by which L-asparaginase activity reached 34.9 μ /ml. Wood shaves as carriers 21 × 10 6 bacterium / ml and efficiency loading of 88.6 μ / ml . One gram of wood shaves produced 88.8 μ / ml while contious Lasparaginase production by recycling immobilized cells could be achieved till 10 cycles with maximum enhancement of 140.2 % after 4 cycles.

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