Abstract

REVIEW ARTICLE: THE APPLICATION OF UHPLC-MS/MS TECHNIQUE IN ANALYTICAL CHEMISTRY

B. Rama Madhuri*, M. Swapna, R. Indu

Abstract

Ultra-High-Performance Liquid Chromatography coupled with tandem Mass Spectrometry (UHPLC-MS/MS) is an analytical powerhouse that combines the rapid, high-resolution separation capabilities of UHPLC with the molecular specificity and sensitivity of MS/MS. This technique is increasingly employed across various fields, such as pharmacology, clinical diagnostics, environmental monitoring, food safety, and forensics, due to its ability to identify and quantify compounds at trace levels in complex matrices. This review article discusses the principles behind the UHPLC-MS/MS technique, its advantages, and the diverse applications that have made it indispensable in modern analytical chemistry.

Keywords: UHPLC-MS/MS, Recent advances in UHPLCMS/ MS, Applications, Analytical chemistry.