Abstract

HPLC-MS, HRMS ANALYSIS OF MICROBIAL ACID FREE, SHORT CHAIN ALKYL SOPHOROSIDES

Vrushali Dengle-Pulate, Parul Dubey, Sunil Bhagwat and Asmita Prabhune*

Abstract

Sophorolipids (SL) are glycolipid biosurfactants abundantly produced from different feedstocks by yeasts and have been widely developed for various applications. The amphipathic structures of sophorolipids imparts to them surfactant type properties. These biosurfactants are readily isolated in high yield, are nontoxic and biodegradable, which makes them industrially interesting as surfactants or emulsifiers. Sophorolipid production was done using glucose as hydrophilic source and lauryl alcohol, as hydrophobic source using Candida bombicola ATCC 22214. Zeta Potential measurements were performed at different pH values to determine the surface charges. Initial identification of structures was performed by MALDI-TOF/TOF. A reverse phase HPLC method combined with electrospray ionization mass detection (HPLC-MS) system was used to identify the structures of individual SL components. Further confirmation of the structures was done using HRMS. Acid free and short chain sophorose lipids were synthesized using primary alcohol. Glycosidic linkage was incorporated directly between the sophorose and hydroxyl function of the lauryl alcohol. Terminal oxidation of the non-functionalized end resulted in novel sophorolipids.

Keywords: Biosurfactants, Sophorolipids, Candida bombicola, lauryl Alcohol, Zeta Potential, HPLC-MS. (SLLA - Sophorolipid obtained using lauryl alcohol).