Abstract

GREEN SYNTHESIS, CHARACTERIZATION OF SILVER NANOPARTICLE AND ITS EVALUATION OF ANTI-OXIDANT, ANTIINFLAMMATORY AND ANTI-MICROBIAL ACTIVITY OF POLYHERBAL GEL FROM AQUEOUS EXTRACT OF DIFFERENT PARTS OF CENTELLA ASIATICA, CURCUMA LONGA, PIPER NIGRUM AND CITRU

Muhammed Hashim Hussain and Dr. M. Siva Ganesh*

Abstract

Green synthesis is an important process for synthesizing nanoparticles through medicinal plant sources. For the synthesis of silver nanoparticles (AgNPs), an economically affordable and “green" approach has been concluded, which has been studied using UV-Visible spectroscopy. The aim of the present study was to evaluate the biosynthesis of silver nanoparticle (AgNPs) using polyherbal formulation from the aqueous extract of four different plant parts from Centella asiatica, Piper nigrum, Curcuma longa and Citrus sinensis. Characterization of AgNPs was performed using UV–visible spectroscopy and FT-IR analysis. From this experiment that conducted on the polyherbal gel that made from the leaves of Centella asiatica, the seed of Piper nigrum, the rhizome part of Curcuma longa and the peel of Citrus sinensis shows a greater pharmacological effect in various aspects. The aqueous extract of each of the plants has the maximum phytochemicals when compared to other solvents such as in ethanol and methanol. The silver nanoparticles were incorporated to the polyherbal gel and its confirmation was done through UV VIS and FTIR analysis. The UV VIS an alysis shows a maximum absorbance at 429nm which confirms the synthesis of silver nanoparticles in the gel. The SOD activity in antioxidant assay gives GAE =127.524μg/ml, AAE=312.96μg/ml for the FRAP activity and 17.20% scavenging activity in DPPH assay. through all of these assays it can be confirmed that it has a good antioxidant activity. The anti inflammatory activity was calculated through the albumin d enaturation method and the result obtained was 40.90% which was excellent anti inflammatory activity. The anti microbial activity was confirmed through the well diffusion method which shows it has higher anti bacterial activity than the disc ampicillin. Th e disc has a zone of inhibition of 19mm while it as 32 for the polyherbal gel. in the three different strains E. coli, pseudomonas aeruginosa and staphylococcus aureus the result was same so that it can be confirmed that it has effect against both the gram negative as well as the gram positive bacteria. The anti fungal test was done on Aspergillus flavus the result obtained was 12mm zone of inhibition and the control fluconazole had 22mm zone of inhibition. The silver nanoparticle incorporated polyherbal ge l had a significant anti oxidant, anti inflammatory as well as the anti microbial activity.

Keywords: Silver Nano Gel, Quercetin , Gallic UV VISIBLE Spectroscopy , FT IR Antioxidant, DPPH, SOD, FRAP, Anti Inflammatory Albumin Denaturation, Antimicro bial Assay Well diffusion method.