Abstract

NEPHROPROTECTIVE SCREENING OF DELONIX REGIA FLOWERS AND LEAVES AGAINST CISPLATIN-INDUCED NEPHROTOXICITY IN FEMALE WISTAR RATS

Muhammad Zishan*, Anjana Sharma and Zubair Alam

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Abstract

Objective: To evaluate the nephroprotective effect of Ethanolic extract of Delonix regia leaves and flowers against cisplatin-induced nephrotoxicity in female Wistar rats. Methods: Nephrotoxicity was induced by Cisplatin at 7mg/kg body weight through the intraperitoneal route in rats. The nephroprotective activity of Ethanolic extract of Delonix regia leaves and flowers (Leaves 200mg/kg, Flowers 200mg/kg, Leaves + Flowers 200mg/kg at 1:1) included a nephrological source. Oxidative stress parameters like GSH and MDA and Biochemical parameters like BUN, SCr, serum uric acid, and total protein were determined and analyzed using one-way ANOVA followed by Tukey's test. Results: The flowers and leaves extract have anti-oxidant activity due to the presence of Gallic acid and flavonoids. The renal function markers like BUN, SCr, and Serum uric acid were found to be decreased significantly by Delonix regia flowers and a combination of leaves and flowers extract (at 1:1) treatment. Conclusion: In conclusion, the presence of Gallic acid, flavonoid, and vitamin E in Delonix regia flowers is likely responsible for the plant's nephroprotective properties. The flower extract of the plant and the combination of leaves and flower extract at 1:1 concentration have anti-oxidant and nephroprotective potential due to the presence of flavonoids and Gallic acid. The administration of Ethanolic extract of Delonix regia flowers provides a prefer able Lowering of plasma creatinine and blood urea nitrogen and serum uric acid levels as compared to the combination of leaves and flowers extract at 1:1 and leaves extract 200mg/kg.

Keywords: Nephrotoxicity, Delonix regia, Cisplatin, Kidney.